Alzet osmotic pumps have been a part of successful research for more than 40 years. These minipumps provide researchers with a method for controlled and continuous agent delivery in vivo. Microosmotic pumps for continuous release of the tyrosine. This video demonstrates subcutaneous and intraperitoneal implantations, intravenous infusion via the external jugular vein, localized administration into the central nervous system. Alzet osmotic pumps are miniature, implantable pumps used for research in mice. Watch this short video to learn how alzet osmotic pumps work to enable reliable and continuous delivery. Implantation of osmotic pumps in rodents university of kentucky.
Subcutaneous angiotensin ii infusion using osmotic pumps. This video article jove describes a method for targeted administration of drugs to the central nervous system by either continuous delivery using alzet osmotic pumps or performing bolus injections into the right lateral ventricle of mice. This video describes the subcutaneous and intraperitoneal. Mini osmotic pumps alzet 2001, 2004, 2006 or micro osmotic pumps alzet 1004. This in vitro testing method gives a good measure of reproducibility over time, both within pumps and. A filling needle is included in the box with osmotic pumps. Watch the above overview video to learn more about the technology, benefits, and. Alzet resource videos osmotic pump mechanism on vimeo. Oral osmotic drug delivery system classification of osmotic dds 8. Osmotic pumps can be used for systemic administration. Osmotic pumps continuously deliver compounds at a constant rate into small animals. Alzet osmotic pumps are miniature infusion systems that deliver test agents at. To examine the relationship between aldosterone and myocardial fibrosis, collagen volume fraction of the left and right ventricles were analyzed by videodensitometry of sirius red stained tissue in the following rat models.
Durect corporation alzet osmotic pump model 1002 alzet. This video describes the inner parts of an alzet pump and how they operate to enable continuous delivery of experimental agents. Alzet pumps have been used to target delivery to a wide variety of sites including the spinal cord, spleen, liver, organ or tissue transplants, and wound. Preparation and implantation alzet osmotic pumps sop9 osmotic pumps manufactured by alza corporation palo alto, ca are designed for constant rate delivery of test solutions over a. Alzet pumps are a simple, convenient and reliable method for continuous dosing of laboratory animals, including mice and rats. They offer a simple, convenient and reliable alternative to repetitive injections, which require frequent animal handling. Durect manufactures and sells alzet osmotic pumps and accessories on a worldwide basis. In addition, durect has a video available on cd or for download that demonstrates surgical techniques and special applications for the use of alzet osmotic pumps. Alzet pumps can also be used for targeted delivery, where the effects of a drug or test agent are localized in a particular tissue or organ, by means of a catheter. Durect corporation alzet osmotic no 1004 pump alzet. As the core absorbs water, it expands in volume, which pushes the drug solution out. In addition, durect has a video available on cd or for download that demonstrates surgical techniques and special applications for the use of alzet osmotic. This 20 minute video describes the alzet pump mechanism, and the advantages of continuous infusion over frequent injections. Articular cavity, blood vessels, bone, brain solid tissue, brain ventricles, ear, eye, muscle, nerves, spinal cord, stomach, and tumors.
Longterm morphine delivery via slow release morphine. Over 12,000 publications attest to the reliability of alzet pumps in preclinical research. Plasma concentration, analgesia, and naloxoneprecipitated withdrawal. Checklist and tips for successful use of alzet osmotic pumps. This video describes proper filling of the alzet pump and and surgical techniques for implantation in rats and mice including subcutaneous and interperitoneal. After filling with compund solution, implantation can be performed in just minutes. This video provides an overview of alzet pump overview on vimeo.
This video shows the parts of the pump and describes how the pump operates to enable continuous delivery of experimental agents. Alzet pumps are small, implantable devices for continuous dosing of unrestrained lab animals. Download article pdf download research materials list. Empty reservoir within the core of the pump is filled with the drug or hormone solution to be delivered and is surrounded by salt chamber with impermeable layer between them it is an implantable osmotic pumps for laboratory animal the pump are used to. Subcutaneous neurotrophin 4 infusion using osmotic pumps or direct muscular injection enhances aging rat laryngeal muscles richard d. Alzet customer service will contact you by the next business day to confirm your order and to supply pricing information. Miniosmotic pumps represent an ideal system for prolonged direct drug delivery into the brain by circumventing both low accessibility to the brain and also peaks of drug concentration, as the delivery of the drug happens directly into a targeted place in the brain and at a fixed flow rate determined by the pump model that is chosen 2,6,7. Alzet pumps deliver by the process of osmotic displacement. Subcutaneous angiotensin ii infusion using osmotic pumps induces. This video describes the subcutaneous and intraperitoneal implantation procedures. Alzet osmotic pumps implantable infusion pumps for. To view the presentation full screen simply click the square icon located in the bottomright corner of the video. Watch this short video for an overview of the alzet technology, benefits and applications. Alzet pumps have been used successfully in experimental biology research for pharmacokinetic analysis, behavior and growth research, physiology, and other applications requiring a precise drug delivery system.
This article introduces a standard protocol used to induce aortic aneurysms via subcutaneous infusion of angiotensin ii angii from implanted osmotic pumps. To achieve widespread gene knockdown or splicing in the cns of mice, the asos can be delivered into the brain using two separate routes of administration, both of which we demonstrate in the video. Download our whitepaper, advancing cancer treatments with alzet. We will continue to respond to the demands for new technology and commit to provide products that assist you.
Free access to all jove education videos until june 15. You may order a complimentary surgical procedures video. You may wish to print this form before submitting your. Durect estimates the pumping rate of alzet osmotic pumps by measuring their pumping rate in vitro in 0. Implantation of miniosmotic pumps and delivery of tract. Alzet osmotic pumps have been a part of successful research for over 40 years. The nominal performance is a target for all pumps manufactured. Maximize therapeutic efficacy and reduce adverse effects. Osmotic pumps release drug at a rate that is independent of the ph and hydrodynamics of the dissolution medium. Longterm morphine delivery via slow release morphine pellets or osmotic pumps. Specify the model, order number and quantity of pumps you require. Tips for successful use this webinar will provide researchers with insider tips everyone should know for planning and executing their animal dosing studies with alzet pumps. You may wish to print this form before submitting your order so you have a copy for your records.
Find the durect media kit here, including logos and press contacts. As the core absorbs water, it expands in volume, which pushes the drug solution out through the delivery ports. Lab animals this video shows lab animals implanted with alzet pumps, and describes how alzet pumps improve animal welfare by reducing the stress caused by repetitive injections and frequent animal handling. Video article demonstrating icv delivery to the mouse cns. Individual lots of pumps may vary from this target within limits. Osmotic pumps consist of an inner core containing drug and osmogens, coated with a semipermeable membrane. Preparation of alzet osmotic pumps for subcutaneous implantation reagentssupplies 1. We market the alzet product line directly in the u. Osmotic pumps are miniature infusion pumps for the continuous dosing of laboratory animals. Osmotic drug delivery system as a part of modified release. Subcutaneous angiotensin ii infusion using osmotic pumps induces aortic aneurysms in mice hong lu 1, deborah a.
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